@article{Rezende_José Galvão Chagas_Perez de Carvalho_Fagundes de Carvalho_Vilar Fernandes_Perez de Carvalho dos Santos_2022, title={Antifungal action of essential oils on Colletotrichum musae in vitro}, volume={14}, url={https://agrogeoambiental.ifsuldeminas.edu.br/index.php/Agrogeoambiental/article/view/1715}, DOI={10.18406/2316-1817v14n120221715}, abstractNote={<p><span style="font-weight: 400;">Anthracnose is a phytosanitary problem affecting banana producers. It is a major postharvest disease and is mainly controlled by the use of fungicides. As the search for healthy foods free of chemical residues has increased among consumers, the objective of this study was to evaluate the</span><em><span style="font-weight: 400;"> in vitro</span></em><span style="font-weight: 400;"> antifungal efficacy of different concentrations of essential oils on mycelial growth of </span><em><span style="font-weight: 400;">Colletotrichum musae</span></em><span style="font-weight: 400;">, the causal agent of anthracnose in banana fruits. The essential oils used were citronella grass (</span><em><span style="font-weight: 400;">Cymbopogon nardus</span></em><span style="font-weight: 400;">), lemongrass (</span><em><span style="font-weight: 400;">Cymbopogon citrates</span></em><span style="font-weight: 400;">), clove (</span><em><span style="font-weight: 400;">Syzygium aromaticum</span></em><span style="font-weight: 400;">), and basil (</span><em><span style="font-weight: 400;">Ocimum basilicum</span></em><span style="font-weight: 400;">). The essential oils were added to a potato dextrose agar medium at concentrations of 0, 0.5, 1.0, 1.5, 2.0,and 2.5 μLmL</span><span style="font-weight: 400;">−1</span><span style="font-weight: 400;"> and distributed in 90 mm diameter Petri dishes. Mycelium discs from the fungal colony, grown for seven days, were transferred to Petri dishes containing the oils and incubated at 25°C with a photoperiod of 12 h (light/dark) cycle for eight days. Colony growth was monitored daily to determine the percentage, index of inhibition of mycelial growth, and average diameter of the colonies. The experimental design consisted of randomized blocks with six different extract concentrations with replicates (n = 4). Each oil extract was then evaluated separately. The essential oils from citronellagrass, lemongrass, and basil inhibited mycelial growth to the proportion that the oil concentration increased. The total inhibition of the pathogen occurred at a concentration of 1.5 µLmL</span><span style="font-weight: 400;">−1 </span><span style="font-weight: 400;">using citronellagrass and lemongrass and 2.5 µLmL</span><span style="font-weight: 400;">−1 </span><span style="font-weight: 400;">using basil. However, essential oils from clove completely inhibited the mycelial growth of </span><em><span style="font-weight: 400;">C. musae </span></em><span style="font-weight: 400;">at a concentration of 0.5 µLmL</span><span style="font-weight: 400;">−1</span><span style="font-weight: 400;">. The tested essential oils showed </span><em><span style="font-weight: 400;">in vitro</span></em><span style="font-weight: 400;"> antifungal activity against </span><em><span style="font-weight: 400;">C. musae</span></em><span style="font-weight: 400;"> at different concentrations. However, essential oils from clove are unique in exhibiting a better inhibitory effect on mycelial growth at lower concentrations.</span></p>}, number={1}, journal={Revista Agrogeoambiental}, author={Rezende, Dalilla Carvalho and José Galvão Chagas, Yuri and Perez de Carvalho, Hebe and Fagundes de Carvalho, Beatriz and Vilar Fernandes, Ítalo and Perez de Carvalho dos Santos, Ana Carolina}, year={2022}, month={set.}, pages={e20221715} }