In vitro addition of DHA and IGF-I increases the progressive motility of cryopreserved stallion semen

Daiane Moreira Silva, Shauna Ann Holden, José Camisão de Souza, Sean Fair

Resumo


High seminal quality is important to achieve acceptable pregnancy rate following artificial
insemination with cryopreserved semen in the equine industry. Docosahexaenoic acid (DHA) is an
omega-3-polyunsaturated acid, which improves the integrity of the spermatozoa membrane during
temperature changes. Insulin-like growth factor-I (IGF-I) is a protein hormone that helps mainly
glucose to enter spermatozoa and it is an antioxidant. The aim of this study was to assess the effect
of the in vitro addition of DHA in combination with IGF-I to frozen-thawed stallion semen. Three
ejaculates from each of three Irish Sport Horse stallions were collected, the gel fraction was removed
and semen was diluted in a 1:1 ratio using extender, centrifuged (1.000 g) for 10 minutes at 32 °C
and ressuspended to 100 x 106 spermatozoa/mL in freezing extender. Semen was cooled to 4 °C,
packed into 0.5 mL straws, frozen and stored under liquid nitrogen at -196 °C. Straws were thawed
at 37 °C for 30 seconds, semen was diluted to 25 x 106 spermatozoa/mL and split in four treatments
adding 0 or 1 ng of DHA /mL and 0 or 100 ng of IGF-I /mL: DHA0, DHA0 + IGF-I, DHA1 and DHA1
+ IGF-I. Semen was incubated at 32 °C and after 30 minutes, total motility (TM), rapid progressive
motility (PM), viability and acrosome integrity were assessed. After 60 and 120 minutes, TM and PM
were assessed again. Post-thawed PM was higher (P < 0.05) when DHA1 + IGF-I was added, but
there was no effect of the addition of DHA and IGF-I to TM, viability or acrosome integrity (P > 0.05).


Keywords: Equine. Spermatozoa. Omega-3. Antioxidant. Hormone.


Palavras-chave


Equine. Spermatozoa. Omega-3. Antioxidant. Hormone.

Texto completo:

ENGLISH

Referências


Barakat, I. A., Danfour, M. A., Galewan, F. A., Dkhil, M. A. Effect of various concentrations of caffeine, pentoxifylline and kallikrein on hyperactivation of frozen bovine semen. Biomed Res Int, 2015. doi: 10.1155/2015/948575.

Blommaert, D., Franck, T., Donnay, I., Lejeune, J. P., Detilleux, J., Serteyn, D. Substitution of egg yolk by a cyclodextrin-cholesterol complex allows a reduction of the glycerol concentration into the freezing medium of equine sperm. Cryobiology, v. 72, p. 27-32, 2016.

Brinsko, S. P., Varner, D. D., Love, C. C., Blanchard, T. L., Day, B. C., Wilson, M. E. Effect of feeding a DHA-enriched nutriceutical on the quality of fresh, cooled and frozen stallion semen. Theriogenology, v. 63, p. 1519-1527, 2005.

Champion, Z. J., Vickers, M. H., Gravance, C. G., Breier, B. H., Casey, P. J. Growth hormone or insulin-like growth factor-I extends longevity of equine spermatozoa in vitro. Theriogenology, v. 57, p. 1793-1800, 2002.

Chanapiwat, P., Kaeoket, K., Tummaruk, P. Effects of DHA-enriched hen egg yolk and L-cysteine supplementation on quality of cryopreserved boar semen. Asian J Androl, v. 11, p. 600-608, 2009.

De Ita, J. R., Castilla-Cortázar, I., Aguirre, G. A., Sánchez-Yago, C., Santos-Ruiz, M. O., Guerra-Menéndez, L., Martín-Estal, I., García-Magariño, M., Lara-Díaz, V. J., Puche, J. E., Muñoz, U. Altered liver expression of genes involved in lipid and glucose metabolism in mice with partial IGF-I deficiency: an experimental approach to metabolic syndrome. J Transl Med, v. 13, p. 1-13, 2015.

Henricks, D. M., Kouba, A. J., Lackey, B. R., Boone, W. R., Gray, S. L. Identification of insulin-like growth factor-I in bovine seminal plasma and its receptor on spermatozoa: influence on sperm motility. Biol Reprod, v. 59, p. 330-337, 1998.

Hernandez-Garzón, E., Fernandez, A. M., Perez-Alvarez, A., Genis, L., Bascuñana, P., Fernandez de la Rosa, R., Delgado, M., Angel Pozo, M., Moreno, E., McCormick, P. J., Santi, A., Trueba-Saiz, A., Garcia-Caceres, C., Tschöp, M. H., Araque, A., Martin, E. D., Torres Aleman I. The insulin-like growth factor-I receptor regulates glucose transport by astrocytes. Glia, doi: 10.1002/glia.23035, 2016.

Kaeoket, K., Sang-urai, P., Thamniyom, A., Chanapiwat, P., Techakumphu, M. Effect of docosahexaenoic acid on quality of cryopreserved boar semen in different breeds. Reprod Domest Anim, v. 45, p. 458-463, 2010.

Kaka, A., Haron, W., Yusoff, N., Khumran, A. M., Sarsaifi, K., Behan, A. A., Kaka, U., Memon, A. A., Ebrahimi, M. Effect of docosahexanoic acid on quality of frozen-thawed bull semen in BioXcell extender. Reprod Fertil Develop, 2015. doi: 10.1071/RD15089.

Kiernan, M., Fahey, A. G., Fair, S. The effect of the in vitro supplementation of exogenous long-chain fatty acids on bovine sperm cell function. Reprod Fertil nd Develop, 25: 947-54, 2013.

Lackey, B. R., Gray, S. L., Henricks, D. M. Measurement of leptin and insulin-like growth factor-I in seminal plasma from different species. Physiol Res, v. 51, p. 309-311, 2002.

Loomis, P. R., Graham, J. K. Commercial semen freezing: individual male variation in cryosurvival and the response of stallion sperm to customized freezing protocols. Anim Reprod Sci, v. 105, p. 119-28, 2007.

Macpherson, M. L., Simmen, R. C., Simmen, F. A., Hernandez, J., Sheerin, B. R., Varner, D. D., Loomis, P., Cadario, M. E., Miller, C. D., Brinsko, S. P., Rigby, S., Blanchard, T. L. Insulin-like growth factor-I and insulin-like growth factor-2 binding protein-2 and -5 in equine seminal plasma: association with sperm characteristics and fertility. Biol Reprod, v. 67, p. 648-654, 2002.

Makarevich, A. V., Spalekova, E., Olexikova, L., Kubovicova, E., Hegedusova, Z. Effect of insulin-like growth factor-I on functional parameters of ram cooled-stored spermatozoa. Zygote, v. 22, p. 305-313, 2014.

Martínez-Soto, J. C., Landeras, J., Gadea, J. Spermatozoa and seminal plasma fatty acids as predictors of cryopreservation success. Andrology, v. 1, p. 365-375, 2013.

Mendez, M. F., Zangeronimo, M. G., Rocha, L. G., Faria, B. G., Pereira, B. A., Fernandes, C. D., Chaves, B. R., Murgas, L. D. S., Sousa, R. V., Effect of the addition of IGF-I and vitamin E to stored boar semen. Animal, v. 7, p. 793-798, 2013.

Milani, C., Fontbonne, A., Sellem, E., Stelletta, C., Gérard, O., Romagnoli, S. Effect of post-thaw dilution with caffeine, pentoxifylline, 2'-deoxyadenosine and prostatic fluid on motility of frozen-thawed dog semen. Theriogenology, v. 74, p. 153-64, 2010.

Moraes, E. A., Matos, W. C., Graham, J. K., Ferrari, W. D. Jr. Cholestanol-loaded-cyclodextrin improves the quality of stallion spermatozoa after cryopreservation. Anim Reprod Sci, v. 158, p. 19-24, 2015.

Morrel, J. M., Stuhtmann, G., Meurling, S., Lundgren, A., Winblad, C., Macias Garcia, B., Johannisson, A. Sperm yield after ingle layer centrifugation with Androcoll-E is related to the potential fertility of the original ejaculate. Theriogenology, v. 81, p. 1005-1011, 2014.

Murphy, C., Holden, S. A., Murphy, E. M., Cromie, A. R., Lonergan, P., Fair, S. The impact of storage temperature on the sperm number on the fertility of liquid-stored bull semen. Reprod Fertil Develop, 2015. doi: 10.1071/RD14369.

Nasiri, A. H., Towhidi, A., Zeinoaldini, S. Combined effect of DHA and -tocopherol supplementation during bull semen cryopreservation on sperm characteristics and fatty acid composition. Andrologia, v. 44, p. 550-555, 2012.

Padilha, R. T., Magalhães-Padilha, D. M., Cavalcante, M. M., Almeida, A. P., Haag, K. T., Gastal, M. O., Nunes, J. F., Rodrigues, A. P., Figueiredo, J. R., Oliveira, M. A., Effect of insulin-like growth factor-I on some quality traits and fertility of cryopreserved ovine semen. Theriogenology, v. 78, p. 907-913, 2012.

Parks, J. E., Lynch, D. V. Lipid composition and thermotrophic phase behavior of boar, bull, stallion and rooster sperm membranes. Cryobiology, v. 29, p. 255-266, 1992.

Peña, F. J., García, B. M., Samper, J. C., Aparicio, I. M., Tapia, J. A., Ferrusola, C. O.

Dissecting the molecular damage to stallion spermatozoa: the way to improve current cryopreservation protocols. Theriogenology, v. 76, p. 1177-1186, 2011.

Schmid-Lausigk, Y., Aurich, C. Influences of a diet supplemented with linseed oil and antioxidants on quality of equine semen after cooling and cryopreservation during winter. Theriogenology, v. 81, p. 966-973, 2014.

Selvaraju, S., Krishnan, B. B., Archana, S. S., Ravindra, J. P. IGF-I stabilizes sperm membrane proteins to reduce cryoinjury and maintain post-thaw sperm motility in buffalo (Bubalus bubalis) spermatozoa. Cryobiology, v. 73, p. 55-62, 2016.

Selvaraju, S., Nandi, S., Subramani, T. S., Raghavendra, B. S., Rao, S. B., Ravindra, J. P. Improvement in buffalo (Bubalus bubalis) spermatozoa functional parameters and fertility in vitro: effect of insulin-like growth factor-I. Theriogenology, v. 73, p. 1-10, 2010.

Selvaraju, S., Reddy, I. J., Nandi, S., Rao, S. B., Ravindra, J. P. Influence of IGF-I on buffalo (Bubalus bubalis) spermatozoa motility, membrane integrity, lipid peroxidation and fructose uptake in vitro. Anim Reprod Sci, v. 113, p. 60-70, 2009.

Shin, S. M., Kim, S., Hong, J. G., Kim, Y. J. IGF-I improves mitochondrial membrane potential during hypothermic storage of canine spermatozoa. J Vet Med Sci, v. 76, p. 1065-1067, 2014.

Silva, D. M., Holden, S. A., de Souza, J. C., Fair, S. In vitro addition of docosahexaenoic acid improves the quality of cooled but not frozen-thawed stallion semen. Reprod Fertil Develop, 2017. doi: 10.1071/RD16473.

Silva, D. M., Zangeronimo, M. G., Murgas, L. D. S, Rocha, L. G., Chaves, B. R., Pereira, B. A., Cunha, E. C. Addition of IGF-I to storage-cooled boar semen and its effect on sperm quality. Growth Horm IGF Res, v. 21, p. 325-330, 2011.

Towhidi, A., Parks, J. E. Effect of n-3 fatty acids and α-tocopherol on post-thaw parameters and fatty acid composition of bovine sperm. J Assist Reprod Genet, v. 29, p. 1051-1056, 2012.

Travascio, F., Elmasry, S., Asfour, S. Modeling the role of IGF-I on extracellular matrix biosynthesis and cellularity in intervertebral disc. J Biomech, v. 47, p. 2269-2276, 2014.

Wu, Z., Zheng, X., Luo, Y., Huo, F., Dong, H., Zhang, G., Yu, W., Tian, F., He, L., Chen, J. Cryopreservation of stallion spermatozoa using different cryoprotectants and combinations of cryoprotectants. Anim Reprod Sci, v. 163, p. 75-81, 2015.




DOI: http://dx.doi.org/10.18406/2316-1817v11n220191257

Apontamentos

  • Não há apontamentos.




Direitos autorais 2019 Daiane Moreira Silva, Shauna Ann Holden, José Camisão de Souza, Sean Fair

Rev. Agrogeoambiental, Pouso Alegre, MG, Brasil. e-ISSN: 2316-1817

Licença Creative Commons

A Revista Agrogeoambiental está licenciada por uma Licença Creative Commons - Atribuição 4.0 Internacional.