In vitro addition of DHA and IGF-I increases the progressive motility of cryopreserved stallion semen

Daiane Moreira Silva, Shauna Ann Holden, José Camisão de Souza, Sean Fair


High seminal quality is important to achieve acceptable pregnancy rate following artificial
insemination with cryopreserved semen in the equine industry. Docosahexaenoic acid (DHA) is an
omega-3-polyunsaturated acid, which improves the integrity of the spermatozoa membrane during
temperature changes. Insulin-like growth factor-I (IGF-I) is a protein hormone that helps mainly
glucose to enter spermatozoa and it is an antioxidant. The aim of this study was to assess the effect
of the in vitro addition of DHA in combination with IGF-I to frozen-thawed stallion semen. Three
ejaculates from each of three Irish Sport Horse stallions were collected, the gel fraction was removed
and semen was diluted in a 1:1 ratio using extender, centrifuged (1.000 g) for 10 minutes at 32 °C
and ressuspended to 100 x 106 spermatozoa/mL in freezing extender. Semen was cooled to 4 °C,
packed into 0.5 mL straws, frozen and stored under liquid nitrogen at -196 °C. Straws were thawed
at 37 °C for 30 seconds, semen was diluted to 25 x 106 spermatozoa/mL and split in four treatments
adding 0 or 1 ng of DHA /mL and 0 or 100 ng of IGF-I /mL: DHA0, DHA0 + IGF-I, DHA1 and DHA1
+ IGF-I. Semen was incubated at 32 °C and after 30 minutes, total motility (TM), rapid progressive
motility (PM), viability and acrosome integrity were assessed. After 60 and 120 minutes, TM and PM
were assessed again. Post-thawed PM was higher (P < 0.05) when DHA1 + IGF-I was added, but
there was no effect of the addition of DHA and IGF-I to TM, viability or acrosome integrity (P > 0.05).

Keywords: Equine. Spermatozoa. Omega-3. Antioxidant. Hormone.


Equine. Spermatozoa. Omega-3. Antioxidant. Hormone.

Texto completo:



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Direitos autorais 2019 Daiane Moreira Silva, Shauna Ann Holden, José Camisão de Souza, Sean Fair

Rev. Agrogeoambiental, Pouso Alegre, MG, Brasil. e-ISSN: 2316-1817

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