Phenolic content, antibacterial and antioxidant activities of herbal infusions
DOI:
https://doi.org/10.18406/2316-1817v13n320211614Palavras-chave:
Teas. C. sylvestris. M. nigra. M. oleifera. H. sabdariffa.Resumo
Natural products derived from plants are considered the oldest medicine in the world. Teas are an accessible source of antioxidant compounds, especially polyphenols that impart flavor, aroma and pharmacological properties. Considering the context of using natural products for health maintenance, the present study aimed to analyze the content of total phenolic compounds, antibacterial and antioxidant activities of infusions using Morus nigra, Casearia sylvestris, Hibiscus sabdariff and Moringa oleifera commercialized in Muzambinho/MG. The infusions were prepared by adding 100 mL of distilled water at approximately 100ºC over 1 g of herb at room temperature for 10 minutes. The analysis of total phenolic compounds was performed using Folin-Ciocalteau. For the analysis of antioxidant activity, the free radical DPPH was used and the results were expressed as a percentage of scavenging activity. For the analysis of antibacterial activity, the microdilution in broth assay was performed in order to find the minimum inhibitory concentrations. It was possible to conclude that the infusion with the highest levels of phenolic compounds were C. sylvestris and M. oleifera, while those that showed a greater antioxidant activity were M. oleifera and M. nigra. As for antibacterial activity, all infusions were able to inhibit E. coli, especially the infusion of C. sylvestris, which was also able to inhibit S. aureus. Although all of the herbs showed antioxidant and antibacterial activities, and also phenolic compounds, C. sylvestis was the herb that stood out in this work because of its phenolic composition and antibacterial activity. In sum, it is concluded that future work is needed to elucidate the exact chemical composition of the infusions and other biological activities.Referências
AMANZE, E. K.; NWANKPA, U. D.; UDEKWU, C. E.; OGBONNA, H. N.; NWOKAFOR, C. V.; UDENSI, C. G. Antibacterial activity of Moringa oleifera root bark extract against some pathogenic organisms. Asian Journal of Immunology, v. 4, n. 3, 2020.
AURELIO, D.; EDGARDO, R. G.; NAVARRO-GALIND, S. Thermal kinetic degradation of anthocyanins in a roselle (Hibiscus sabdariffa L. cv. ‘Criollo') infusion, International Journal of Food Science and Technology, v. 43, n. 2, p. 322–325, 2008.
BAGHERI, G.; MARTORELL, M.; RAMIREZ-ALARCON, K.; SALEHI, B.; SHARIFI-RAD, J. Phytochemical screening of Moringa oleifera leaf extracts and their antimicrobial activities. Cellular and Molecular Biology, 2020. Doi: 10.14715/cmb/2019.66.1.3
BRAND-WILLIAMS; CUVELIER, M. E.; BERSET, C. Use of a Free Radical Method to Evaluate Antioxidant Activity. Food Science and Technology, v. 28, p. 25–30, 1995.
BUENO, P. C. P.; PEREIRA, F. M. V.; TORRES, R. B.; CAVALHEIRO, A. J. Development of a comprehensive method for analyzing clerodane-type diterpenes and phenolic compounds from Caesaria sylvestris Swartz (Salicaceae) based on ultra-high performance liquid chromatography combined whit chemometric tools. Journal of Separation Science, 2015. Doi: 10.1002/jssc.201401421
CLAUDINO, J. C.; SACRAMENTO, L. V. S.; KOCH, I.; SANTOS, H. A.; CAVALHEIRO, A. J.; TININIS, A. G.; SANTOS, A. G. Evaluation of morpho-anatomical and chemical differences between varieties of the medicinal plant Casearia sylvestris Swartz. Annals of the Brazilian Academy of Sciences, v. 85, n. 4, 2013. Doi: http://dx.doi.org/10.1590/0001-3765201393812.
CLSI. Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically. Vol. 35, n. 2. Wayne, PA: Clinical and Laboratory Standards Institute, 2015.
DA-COSTA-ROCHA, I.; BONNLAENDE, B.; SIEVERS, H.; PISCHEL, I.; HEINRICH, M. Hibiscus sabdariffa L. A phytochemical and pharmacological review. Food Chemistry, v. 165, p. 424-443, 2014.
DHAKAD, A. K.; IKRAM, M.; SHARMA, S.; KHAN, S.; PANDEY, V. V.; SINGH, A. Biological, nutritional, and therapeutic significance of Moringa oleifera Lam. Phytotherapy Research, v. 33, 2019.
ERCISLI, S.; ORHAN, E. Chemical composition of white (Morus alba), red (Morus rubra) and black (Morus nigra) mulberry fruits. Food Chemistry, v. 3, p. 1380-1384, 2007.
EUROMONITOR INTERNATIONAL. Tea in Brazil report. 2021. Available: https://www.euromonitor.com/tea-in-brazil/report. Access Jan. 25th, 2021.
FERNANDES, M. F. G. Composição química e atividades antioxidante e antimicrobiana in vitro dos extratos das folhas de Casearia sylvestris e Lithraea molleoides. 2013. 48 f. Dissertação (Mestrado) - Curso de Ciências Biológicas, Universidade Estadual de Montes Claros, Montes Claros, 2013.
FERREIRA, D. F. Sisvar: a Guide for its Bootstrap procedures in multiples comparisons. Ciência e Agrotecnologia, v. 38, n. 2, 2014.
FOUAD, E. A.; ELNAGA, A. S. M. A.; KANDIL, M. M. Antibacterial efficacy of Moringa oleifera leaf extract against pyogenic bacteria isolated from a dromedary camel (Camelus dromedarius) abscess. Veterinary World, v. 12, n. 6, 2019. Doi: 10.14202/vetworld.2019.802-808.
GODOY, R. C. B.; DELIZA, R.; GHENO, L. B.; LICODIEDOFF, S.; FRIZON, C. N. T.; RIBANI, R. H.; SANTOS, G. G. Consumer perceptions, atitudes and acceptance of new and traditional mate tea products. Food Research International, v. 53, 2013. Doi: http://dx.doi.org/10.1016/j.foodres.2013.02.054.
HALLIWELL, B. Free radicals and antioxidants: Updating a personal view. Nutrition Reviews, v. 70, n. 5, p. 257–265, 2012.
HUSSAIN, F.; RANA, Z.; SHAFIQUE, H.; MALIK, A.; HUSSAIN, Z. Phytopharmacological potential of different species of Morus alba and their bioactive phytochemicals: A review. Asian Pacific Journal of Tropical Biomedicine, v. 7, p 950-956, 2017.
IBRAHIM, D. A.; ALBADANI, R. N. Evaluation of the potential nephroprotective and antimicrobial effect of Camellia sinensis leaves versus Hibiscus sabdariffa (in vivo and in vitro studies). Advances in Pharmacological and Pharmaceutical Sciences, 2014. Doi: https://doi.org/10.1155/2014/389834.
KHAN, N.; MUKHTAR, H. Tea polyphenols in promotion of human health. Nutrients, v. 11, n. 39, 2019. Doi: 10.3390/nu11010039.
MALONGANE, F.; MCGAW, L. J.; MUDAU, F. N. The synergistic potential of various teas, herbs and therapeutic drugs in health improvement: a review. Journal of the Science of Food and Agriculture, v. 97, n. 14, 2017. Doi: 10.1002/jsfa.8472.
MELO, E. A.; MACIEL, M. I. S.; LIMA, V. L. A. G.; NASCIMENTO, R. J. Antioxidant capacity of the fruits. Revista Brasileira de Ciências Farmacêuticas, v. 44, n. 2, 2008. Doi: https://doi.org/10.1590/S1516-93322008000200005.
NASCIMENTO, J. A.; ARAÚJO, K. L. G. V.; EPAMINONDAS, P. S.; SOUSA, A. S.; MAGNANI, M.; SOUZA, A. L.; SOLEDADE, L. E. B.; QUEIROZ, N.; SOUZA, A. G. Ethanolic extracts of Moringa oelifera Lam. Journal of Thermal Analysis and Calorimetry, v. 114, 2013.
PEREIRA, F. G.; MARQUETE, R.; DOMINGOS, L. T.; ROCHA, M. E. N.; FERREIRA-PEREIRA, A.; MANSUR, E.; MOREIRA, D. L. Antifungal activities of the essential oil and its fractions rich in sesquiterpenes from leaves of Casearia sylvestris Sw. Annals of the Brazilian Academy of Sciences, v. 89, n. 4, 2017.
POSWAL, F. S.; RUSSELL, G.; MACKONOCHIE, M.; MACLENNAN, E.; ADUKWU, E. C.; ROLFE, V. Herbal Teas and their health benefits: a scoping review. Plant Foods for Human Nutrition, v. 3, n. 74, 2019. Doi: 10.1007/s11130-019-00750-w.
RAJARAM, S.; JONES, J.; LEE, G. J. Plant-based dietary patterns, plant foods, and age-related cognitive decline. Advances in Nutrition, v. 10, 2019. Doi: https://doi.org/10.1093/advances/nmz081.
RAMOS, D. D.; VIEIRA, M. C.; FORMAGIO, A. S. N.; CARDOSO, C. A. L.; RAMOS, D. D.; CARNEVALI, T. O. Antioxidant activity of Hibiscus sabdariffa L. in function of spacing between plants and organic fertilization. Ciência Rural, v. 41, n. 8, 2011.
REUTER, S.; GUPTA, S. C.; CHATURVEDI, M. M.; AGGARWAL, B. B. Oxidative stress, inflammation, and cancer: How are they linked? Free Radical Biology & Medicine, v. 49, p. 1603–1616, 2010.
RIAZ, G.; CHOPRA, R. A review on phytochemistry and therapeutic uses of Hibiscus sabdariffa L. Biomedicine & Pharmacotherapy, n. 102, 2018. Doi: https://doi.org/10.1016/j.biopha.2018.03.023.
ROCHA, I. C.; BONNLAENDER, B.; SIEVER, H.; PISCHEL, I.; HEINRICH, M. Hibiscus sabdariffa L – a phytochemical and pharmacological review. Food Chemistry, n. 165, 2014. Doi: http://dx.doi.org/10.1016/j.foodchem.2014.05.002.
RODRIGUES, L. E.; MARCELINO, G.; SILVA, G. T.; FIGUEIREDO, P. S.; GARCEZ, W. S.; CORSINO, J.; GUIMARÃES, R. C. A.; FREITAS, K. C. Nutraceutical and medicinal potential of the Morus species in metabolic dysfunctions. International Journal of Molecular Sciences, v. 20, n. 301, 2019. Doi: 10.3390/ijms20020301.
SCHULZE, M. B.; MARTÍNEZ-GONZÁLEZ, M. A.; FUNG, T. T.; LICHTENSTEIN, A. H.; FOROUHI, N. G. Food based dietary patterns and chronic disease prevention. BMJ, n. 361, 2018. Doi: https://doi.org/10.1136/bmj.k2396 .
SINGH, R. K.; SUREJA, K. A.; SINGH, D. Amta and Amti (Hibiscus sabdariffa L.) –Cultural and agricultural dynamics of agrobiodiversity conservation. Indian Journal of Traditional Knowledge, v. 5, n. 1, p. 151-157, 2006.
SINGLETON, V. L.; ORTHOFER, R.; LAMUELA-RAVENTÓS, R. M. Analysis of total phenols and other oxidation substrates and antioxidants by means of folin-ciocalteu reagent. Methods in Enzymology, v. 299, n. 1974, p. 152–178, 1998.
SOUZA, G. R.; OLIVEIRA-JUNIOR, R. G.; DINIZ, T. C.; BRANCO, A.; LIMA-SARAIVA, S. R. G.; GUIMARÃES, A. L.; OLIVEIRA, A. P.; PACHCO, A. G. M.; SILVA, M. G.; MORAES-FILHO, M. O.; COSTA, M. P.; PESSOA, C. Ó.; ALMEIDA, J. R. G. S. Assessment of the antibacterial, cytotoxic and antioxidante activities of Morus nigra L. (Moraceae). Brazilian Journal of Biology, v. 78, n. 2, 2018. Doi: https://doi.org/10.1590/1519-6984.05316.
SOUZA, R. A. M.; OLDONI, T. L. C.; CABRAL, I. S. R.; ALENCAR, S. M. Compostos fenólicos totais e atividade antioxidante de chás comercializados no Brasil. Boletim do Centro de Pesquisa de Processamento de Alimentos, v. 29, n. 2, p. 229-236, 2011.
STOHS, S. J.; HARTMAN, M. J. Review of the safety and efficacy of Moringa oleifera. Phytotherapy Research, v. 29, 2015. Doi: 10.1002/ptr.5325
TALIB, W. H.; AL-ATABY, I. A.; MAHMOD, A. I.; JAWARNECH, S.; KURY, L. T.; AL-YASARI, I. H. The impact of herbal infusion consumption on oxidative stress and cancer: the good, the bad, the misunderstood. Molecules, v. 25, 2020. Doi: https://doi.org/10.3390/molecules25184207.
WANG, W.; LI, X. C.; ALI, Z.; KHAN, I. A. Neolignans from the leaves of Casearia sylvestris Swartz. Helvetica Chimica Acta, v. 93, 2010. Doi: 10.1002/hlca.200900211
WHO – WORLD HEALTH ORGANIZATION. WHO traditional medicine strategy 2014-2023, World Health Oganization. Available: . Access Jan 19th, 2021.
ZANCO, P.; SOUZA, E. F.; BONACORSI, C. Avaliação da atividade antimicrobiana e antioxidante de chás de folhas da amora-preta (Morus nigra). Scientific Electronic Archives, Mato Grosso, v. 10, n. 1, p.150-157, fev. 2017.
ZENI, A. L. B.; MOREIRA, T. D.; DALMAGRO, A. P.; CAMARGO, A.; BINI, L. A.; SIMIONATTO, E. L.; SCHARF, D. R. Evaluation of phenolic compounds and lipid-lowering effect of Morus nigra leaves extract. Anais da Academia Brasileira de Ciências, v. 89, n. 4, 2017. Doi: http://dx.doi.org/10.1590/0001-3765201720160660.
Downloads
Publicado
Como Citar
Edição
Seção
Licença
Copyright (c) 2022 Larissa Ferreira Silva, Amanda Tristão Santini, Carolina Lima Silva, Flávia Apolinário Galera, Ingridy Simone Ribeiro
Este trabalho está licenciado sob uma licença Creative Commons Attribution 4.0 International License.
A Revista Agrogeoambiental é um periódico científico de acesso aberto e gratuito.
A submissão de artigos e demais obras de comunicação científica para a Revista Agrogeoambiental implica plena aceitação pelo autor e pelos coautores da política de direitos autorais abaixo:
● Autor e coautores mantêm os direitos autorais e concedem à revista o direito de primeira publicação, com o trabalho simultaneamente licenciado sob a Licença Creative Commons Attribution, que permite o compartilhamento do trabalho com reconhecimento da autoria e publicação inicial nesta revista.
● Autor e coautores têm permissão e são estimulados a publicar e distribuir seu trabalho on-line (ex.: em repositórios institucionais ou na sua página pessoal) após seu aceite e publicação pela Revista Agrogeoambiental - mantendo o reconhecimento de autoria e publicação inicial nesta revista.
● Autor e coautores declaram que a obra é de autoria deles e responsabilizam-se por sua originalidade e pelas opiniões nela contidas.
● Após aceito e publicado o artigo, autor e coautores autorizam o editor a divulgar em mídias e modalidades de escolha do editor.
● O autor e os coautores resguardam os direitos autorais morais do obra publicada.