Micropropagation of Dalbergia nigra, an endangered Brazilian forest tree

Authors

  • Rodrigo Oliveira Almeida Instituto Federal do Sudeste de Minas Gerais – Campus Muriaé http://orcid.org/0000-0002-7444-0864
  • Sarah Santos da Silva Instituto Federal do Sudeste de Minas Gerais – Campus Rio Pomba
  • Ana Catarina Monteiro Carvalho Mori da Cunha Instituto Federal de Alagoas

DOI:

https://doi.org/10.18406/2316-1817v12n420201478

Keywords:

Jacarandá-da-Bahia. In vitro propagation. Organogenesis.

Abstract

We report in this work an in vitro propagation method for Dalbergia nigra using nodal segments from germinated seeds in vitro. Plant material was cultivated on MS medium supplemented with 6-benzyladeninepurine (BAP: 0 μM, 1.11 μM and 2.22 μM) and Naphthaleneacetic acd (NAA: 0.27 μM), combined with different amounts of subcultures for induction of shoot multiplication. Multiplied shoots were inoculated in MS and ½ MS medium (half strength of MS salts) to root, supplemented with NAA (0 μM and 4 μM). Data were analyzed by non-parametric statistical Kruskal-Wallis test. Best results to shoot multiplication were obtained using 1.11 μM or 2.22 μM of BAP combined 0.27 μM of NAA in multiplication medium and performing three subcultures, providing higher rates of shoots
production per explant (average of 2.24) and high survival rate. Best results for rooting of shoots generated were obtained using a rooting medium containing ½ MS supplemented with 4 μM of NAA, providing a higher percentage of shoot rooting (average of 68%).

Author Biographies

Rodrigo Oliveira Almeida, Instituto Federal do Sudeste de Minas Gerais – Campus Muriaé

Doutor em Biotecnologia

Sarah Santos da Silva, Instituto Federal do Sudeste de Minas Gerais – Campus Rio Pomba

Bacharela em Agroecologia

Ana Catarina Monteiro Carvalho Mori da Cunha, Instituto Federal de Alagoas

Professora doutora

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Published

2021-03-10

How to Cite

Almeida, R. O., da Silva, S. S., & Cunha, A. C. M. C. M. da. (2021). Micropropagation of Dalbergia nigra, an endangered Brazilian forest tree. Revista Agrogeoambiental, 12(4). https://doi.org/10.18406/2316-1817v12n420201478

Issue

Vol. 12 No. 4 (2020)

Section

Artigo Científico

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