Biopolymer Production by Rhizobacteria Associated with Cactaceae
DOI:
https://doi.org/10.18406/2316-1817v18nunico20262061Keywords:
Exopolysaccharides, Biofilms, Caatinga, MicrobiomeAbstract
Bacterial biopolymers, due to their biodegradable nature, have gained prominence as a sustainable alternative to petroleum-derived polymers, driving research aimed at optimizing their production and application across various industrial sectors. In this context, the present study aimed to select native rhizobacteria from the Caatinga biome with the potential to synthesize exopolysaccharides (EPS) and to define the optimal conditions for their production. Bacterial suspensions were inoculated onto sterile filter paper discs placed on an EPS-inducing culture medium. EPS production was determined by the formation of a mucoid layer and confirmed in the presence of absolute ethanol. The isolates exhibiting the highest EPS production were subjected to assays based on a Central Composite Rotational Design (CCRD), varying pH, temperature, and glucose as a carbon source. EPS recovery was evaluated under two conditions: static fermentation and constant agitation. Among the 15 selected isolates, PH9.1 showed the best performance in trial 13 (pH 7.0; temperature 38.5°C; glucose 1%), with a mucoid layer diameter of 2.15 cm, surpassing the others. Furthermore, static fermentation resulted in a higher yield of fresh mass (0.64 g) and dry mass (0.42 g), highlighting it as the most efficient condition. These findings reinforce the biotechnological potential of Caatinga rhizobacteria for sustainable EPS production and support their application in emerging industrial processes.
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